Journal: Stem Cell Research & Therapy

Article Title: Spatio-temporal model of Meox1 expression control involvement of Sca-1-positive stem cells in neointima formation through the synergistic effect of Rho/CDC42 and SDF-1α/CXCR4

doi: 10.1186/s13287-021-02466-8

Figure Lengend Snippet: CDC42 was involved in SDF-1α expression in VSMC and CXCR4 expression in Sca-1+ stem cells mediated by Meox1. The aim of the experiment was to explore possible molecular mechanisms of Meox1-induced SDF-1α expression in VSMC and CXCR4 expression in HUCSCs, using RhoA inhibitor CCG1423, CDC42 blocker ZCL278, or Rac1 inhibitor Azathioprine. A , B Overexpressing Meox1 (Ad-Meox1) increased SDF-1α expressions while knockdown of Meox1 by shRNA (Ad-shMeox1) in VSMCs transfected with treatment of Ad-Meox1 or Ad-shMeox1 for 3 days as determined by western blot ( A ) and semi-quantitative analysis ( B ). Three independent experiments were performed, n = 3, * P < 0.05 vs. VSMC transfected with Ad-Null; # P < 0.05 vs. VSMCs transfected with Ad-Meox1. C , D The SDF-1α expressions in VSMCs transfected with Ad-Meox1, following the addition of RhoA inhibitor CCG1423, CDC42 blocker ZCL278, or Rac1 inhibitor Azathioprine, as determined by western blot ( C ) and semi-quantitative analysis ( D ). Three independent experiments were performed, n = 3, * P < 0.05 vs. VSMCs transfected with Ad-Null; # P < 0.05 vs. VSMCs transfected with Ad-Meox1. E , F CXCR4 expressions in HUCSCs transfected with Ad-Meox1 for 24 h, following the addition of CCG1423, or Azathioprine for 48 h, as determined by western blot ( E ) and semi-quantitative analysis ( F ). Three independent experiments were performed, n = 3, * P < 0.05 vs. HUCSCs treated with Ad-Null; # P < 0.05 vs. HUCSCs treated with Ad-Meox1; & P < 0.05 vs. Ad-Meox1-transfected HUCSCs treated with CDC42 blocker ZCL278

Article Snippet: Primary vascular smooth muscle cells (VSMC) were isolated from aortic artery of Sprague Dawley rats (280–300 g) and cultured as previously described [ ].

Techniques: Expressing, Knockdown, shRNA, Transfection, Western Blot

Journal: Diseases

Article Title: Osteocalcin, Osteopontin and RUNX2 Expression in Patients’ Leucocytes with Arteriosclerosis

doi: 10.3390/diseases9010019

Figure Lengend Snippet: Alkaline phosphatase activity (ALP) in human umbilical vein endothelial cells (HUVEC) and vascular smooth muscle cells (VSMC) cells treated with calcification medium. Note that calcification does not alter ALP activity in HUVEC cells, *** p < 0.001.

Article Snippet: VSMC (vascular smooth muscle cells, Lifeline Cell Technology) were cultured in Ham’s F-12K Medium (Thermo Fisher Waltham, MA, USA) supplemented with 0.05 mg/mL Ascorbic acid (Sigma-Aldrich, St. Louis, MO, USA), 0.01 mg/mL Insulin (Sigma-Aldrich), 0.01 mg/mL Transferrin (SERVA), 10 ng/mL Sodium selenite (Sigma-Aldrich), 0.03mg/mL Endothelial Cell Growth Supplement (ECGS) (Sigma-Aldrich), 10 mM HEPES (Sigma-Aldrich), 10 mMTES (Sigma-Aldrich) and 10% FCS (Biochrom AG).

Techniques: Activity Assay